Journal
METHODS
Volume 29, Issue 1, Pages 42-50Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S1046-2023(02)00289-X
Keywords
live cell microscopy; live chromatin labeling; live chromosome labeling; chromosome territory
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The dynamic organization of eukaryotic genomes in cell nuclei recently came into the focus of research interest. The kinetics of genome dynamics can be addressed only by approaches involving live cell microscopy. Different methods are available to visualize chromatin, specific chromatin fractions, or individual chromosome territories within nuclei of living mammalian cells. Appropriate labeling procedures as well as cell chamber systems and important controls for live cell microscopy are described. (C) 2002 Elsevier Science (USA). All rights reserved.
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