Journal
TRAFFIC
Volume 4, Issue 12, Pages 902-910Publisher
WILEY
DOI: 10.1046/j.1600-0854.2003.00145.x
Keywords
human immunodeficiency virus type 1 (HIV-1); late endosome; multivesicular body (MVB); retroviral assembly and release
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Funding
- NIAID NIH HHS [R01 AI 40338, R01 AI047727, R01 AI 47727] Funding Source: Medline
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI047727, R01AI040338] Funding Source: NIH RePORTER
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HIV-1 buds from the surface of activated T lymphocytes. In macrophages, however, newly formed HIV-1 particles amass in the lumen of an intracellular compartment. Here, we demonstrate by live-cell imaging techniques, by immunocytochemistry and by immuno-electron microscopy that HIV-1 structural proteins, particularly the internal structural protein Gag, accumulate at membranes of the late endocytic compartment in a variety of cell types and not just in monocyte/macrophage-derived cells. Recent biochemical and genetic studies have implicated components of the mammalian vacuolar protein sorting pathway in retroviral budding. Together with those observations, our study suggests that HIV-1 morphogenesis is thoroughly rooted in the endosomal system.
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