Journal
CELL STRESS & CHAPERONES
Volume 15, Issue 6, Pages 819-826Publisher
SPRINGER
DOI: 10.1007/s12192-010-0190-1
Keywords
CCT; CCT-eta; Chaperonin; Wound healing; Alpha smooth muscle actin
Categories
Funding
- Allegheny Singer Research Institute, Allegheny General Hospital
- Pittsburgh Tissue Engineering Institute (PTEI)
- AFIRM [DE014780]
- 3M Fellowship
- [DC 05659]
- [DC 04173]
Ask authors/readers for more resources
We have previously identified the CCT subunit eta as specifically reduced in healing fetal skin wounds by differential display, and observed that this reduction is not seen with any other CCT subunit. We now report the cloning and characterization of the cDNAs for rabbit CCT-eta and its closest evolutionary homolog, CCT-beta. Quantitative examination of CCT-eta and -beta message expression in healing fetal and adult wounds at 12 h post-injury confirms that CCT-eta mRNA is decreased in fetal wound tissues, but actually elevated in adult wound tissues. CCT-beta mRNA, in contrast, remains unchanged in both fetal and adult wound tissues. CCT-eta mRNA remains persistently elevated in healing adult wounds for 28 days following injury, whereas CCT-beta mRNA remains invariant throughout. CCT-eta protein is similarly increased, whereas CCT-beta protein remains unchanged. proportional to-smooth muscle actin (proportional to-SMA), a recognized substrate of CCT known to be important in integumentary wound healing, was also measured over the course of wound healing, and both mRNA and protein levels were elevated throughout the 28 days.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available