Journal
CELL STRESS & CHAPERONES
Volume 13, Issue 3, Pages 275-285Publisher
SPRINGER
DOI: 10.1007/s12192-008-0044-2
Keywords
atherosclerosis; imaging; isotope labeling; radiology; stress proteins
Categories
Ask authors/readers for more resources
Bacterial endotoxins are known as stress factors for endothelial cells. In 20 normocholesterolemic New Zealand White (NZW) rabbits, endothelial stress was induced by intravenous (i.v.) injection of lipopolysaccharide (LPS), while eight NZW rabbits were sham-treated or served as untreated controls. In vivo molecular imaging was performed using co-registered computer tomography and positron emission tomography 24 h after i.v. injection of I-124-labeled monoclonal anti-HSP60 or I-124-radiolabelled isotype control antibodies. Compared to control animals, in vivo images of rabbit aortae revealed significantly increased endothelial binding of I-124-labeled anti-HSP60 antibodies upon LPS, especially at sites of aortal branching. This was confirmed by immunohistochemistry and autoradiography data. Our results showed, as proof-of-principle, that HSP60-expression in normocholesterolemic rabbits is significantly increased after induction of endothelial stress and that non-invasive in vivo molecular imaging of early aortal HSP60-expression using I-124-labeled anti-HSP60 monoclonal antibodies is possible.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available