4.7 Article

Pathways Disrupted in Human ALS Motor Neurons Identified through Genetic Correction of Mutant SOD1

Journal

CELL STEM CELL
Volume 14, Issue 6, Pages 781-795

Publisher

CELL PRESS
DOI: 10.1016/j.stem.2014.03.004

Keywords

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Funding

  1. ALS Therapy Alliance, Project ALS, P2ALS
  2. Angel Fund
  3. Pierre L. de Bourgknecht ALS Research Foundation
  4. Al-Athel ALS Research Foundation
  5. ALS Family Charitable Foundation
  6. NIH/NINDS [1R01NS050557]
  7. NIH/NINDS (NINDS ARRA Award) [RC2-NS070-342]
  8. Target ALS, Project A.L.S., P2ALS
  9. NINDS GO grant [5RC2NS069395-02]
  10. NINDS R24 [1U24NS078736-01]
  11. HHMI
  12. NIH Director's Pioneer Award DP1 [OD006862]

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Althoughmany distinct mutations in a variety of genes are known to cause Amyotrophic Lateral Sclerosis (ALS), it remains poorly understood how they selectively impact motor neuron biology and whether they converge on common pathways to cause neuronal degeneration. Here, we have combined reprogramming and stem cell differentiation approaches with genome engineering and RNA sequencing to define the transcriptional and functional changes that are induced in human motor neurons by mutant SOD1. Mutant SOD1 protein induced a transcriptional signature indicative of increased oxidative stress, reduced mitochondrial function, altered subcellular transport, and activation of the ER stress and unfolded protein response pathways. Functional studies demonstrated that these pathways were perturbed in a manner dependent on the SOD1 mutation. Finally, interrogation of stem-cell-derived motor neurons produced from ALS patients harboring a repeat expansion in C9orf72 indicates that at least a subset of these changes are more broadly conserved in ALS.

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