Journal
JOURNAL OF ELECTRON MICROSCOPY
Volume 52, Issue 4, Pages 429-433Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jmicro/52.4.429
Keywords
anaerobic biofilms; critical point drying method; granular sludge; HMDS treatment; SEM analysis
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We present a fast procedure for scanning electron microscopy (SEM) analysis in which hexamethyldisilazane (HMDS) solvent, instead of the critical point drying, is used to remove liquids from a microbiological specimen. The results indicate that the HMDS solvent is suitable for drying samples of anaerobic cells for examination by SEM and does not cause cell structure disruption.
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