Journal
INTERNATIONAL DAIRY JOURNAL
Volume 13, Issue 7, Pages 509-516Publisher
ELSEVIER SCI LTD
DOI: 10.1016/S0958-6946(03)00073-6
Keywords
Lactobacillus helveticus; peptidase activity; growth phase; growth medium
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The effect of growth phase, growth medium and storage conditions on the peptidase activities of Lactobacillus helveticus DPC 4571 were examined. Cell free extracts (CFEs) were prepared from both mid-log and stationary phase cells grown in either 10% (w/v) reconstituted skim milk (RSM) or MRS pH 5.4 broth. In general, Lb. helveticus DPC 4571 was found to possess a broad range of aminopeptidase, dipeptidase, tripeptidase, endopeptidase and proline-specific peptidase activities. The stability of these peptidase activities in a CFE of Lb. helveticus DPC 4571 stored at 4 C was variable. The specific activities of the majority of these peptidases were significantly (P<0.05) influenced by both growth phase and growth medium. Cells grown to mid-log phase in RSM were found to have the highest level of peptidase activity. (C) 2003 Elsevier Science Ltd. All rights reserved.
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