Journal
CELL STEM CELL
Volume 3, Issue 6, Pages 595-605Publisher
CELL PRESS
DOI: 10.1016/j.stem.2008.11.008
Keywords
-
Categories
Funding
- Natural Sciences and Engineering Research Council of Canada
- Alberta Scholarship Programs
- NIH Director's Innovator Award
- Harvard Stem Cell Institute
- Kimmel Foundation
- V Foundation
Ask authors/readers for more resources
Direct reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) provides an invaluable resource for regenerative medicine, enabling the generation of patient-specific cells of any lineage without the use of embryonic material. A variety of methods exist for iPSC derivation, all reliant upon manipulation of a select group of transcription factors. We compare the currently reported protocols, identify essential steps common to these methods, and suggest minimal criteria for defining fully reprogrammed cells. In addition, specific procedures aimed to optimize reproducible iPSC derivation are presented, with an emphasis on standardization of certain parameters for accurate comparison between independent experiments.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available