Journal
CELL RESEARCH
Volume 21, Issue 12, Pages 1691-1700Publisher
INST BIOCHEMISTRY & CELL BIOLOGY
DOI: 10.1038/cr.2011.173
Keywords
DTF1; siRNA; DNA methylation; transcriptional gene silencing; RdDM
Categories
Funding
- Chinese Ministry of Science and Technology [2011CB812600]
- US National Institutes of Health [R01GM070795]
Ask authors/readers for more resources
RNA-directed DNA methylation (RdDM) is an important de novo DNA methylation pathway in plants. RdDM mediates the transcriptional silencing of many endogenous genomic loci, most of which are transposon related. A forward genetics screen identified DTF1 (DNA-binding transcription factor 1) as a new component for RdDM in Arabidopsis. Loss-of-function mutations in DTF1 release the transcriptional silencing of RdDM target loci and reduce the accumulation of 24-nt small interfering RNAs (siRNAs) from some of the targets. Interestingly, in the dtf1 mutant plants, the release of transcriptional gene silencing at solo-LTR is accompanied by decreased siRNA accumulation but not by reduced DNA methylation. These results suggest that DTF1 is an atypical component of RdDM and has both DNA methylation-dependent and -independent roles in transcriptional gene silencing. We suggest that besides DNA methylation, siRNAs may cause some other uncharacterized epigenetic modifications that lead to transcriptional gene silencing.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available