Journal
JOURNAL OF FISH DISEASES
Volume 39, Issue 2, Pages 175-188Publisher
WILEY-BLACKWELL
DOI: 10.1111/jfd.12345
Keywords
caudal fin; epithelial cells; fibroblast; VHSV; viral tropism; walleye
Funding
- Natural Science and Research Council (NSERC) of Canada
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A cell line, WE-cfin11e, with an epithelial-like morphology was developed from a caudal fin of walleye, Sander vitreus (Mitchill), characterized as distinct from the established walleye caudal fin fibroblast-like cell line, WE-cfin11f, and compared with WE-cfin11f for susceptibility to VHSV IVb. Immunocytochemistry and confocal microscopy were used to localize the intermediate filament protein, vimentin, the tight junction protein, zonula occludens-1 (ZO-1), the extracellular matrix protein, collagen I, and the viral protein, G. Although both cell lines contained vimentin, only WE-cfin11e stained for ZO-1 and only WEcfin11f stained for collagen I. Ascorbic acid increased the accumulation of collagen I and caused the appearance of collagen fibres only in WE-cfin11f cultures. At 14 degrees C, both cell lines produced VHSV IVb, but the infection developed more rapidly in WE-cfin11f. At 4 degrees C, both cell lines became infected with VHSV IVb as judged by the expression of viral proteins, N and G, but only WE-cfin11f produced virus. The results suggest that cold temperatures can modulate viral tropism.
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