Journal
INSECTES SOCIAUX
Volume 50, Issue 2, Pages 199-200Publisher
BIRKHAUSER VERLAG AG
DOI: 10.1007/s00040-003-0662-8
Keywords
monogyne; polygyne; Solenopsis invicta; Gp-9
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Oligonucleotide primers were designed to discriminate between the Gp-9(B) and Gp-9(b) alleles found in monogyne and polygyne colonies of fire ant, Solenopsis invicta Buren. Primers specific for the Gp-9(B) allele produced a 517 bp amplicon and primers, specific for Gp-9(b) allele produced a 423 bp amplicon. When both sets of primers were multiplexed, homozygous monogyne ants produced a single 517 bp amplicon (specific for Gp-9(B)), whereas heterozygous polygyne ants produced one 517 bp amplicon and one 423 bp amplicon (specific for Gp-9(B) and Gp-9(b), respectively) which allowed the Gp-9 alleles to be discerned in a single reaction. This method was tested on ants from 20 monogyne colonies and 20 polygyne colonies and was 100% accurate in discriminating the two forms.
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