Journal
CELL HOST & MICROBE
Volume 10, Issue 5, Pages 515-526Publisher
CELL PRESS
DOI: 10.1016/j.chom.2011.09.012
Keywords
-
Categories
Funding
- National Institutes of Health (NIH) [K99-CA-137860-01A1/R00-CA-137860-02, R01-AI067968, T32-CA009111, GM073047, MH08442, RC1CA145442]
- Charles Revson, Jr. Foundation
- Starr Foundation
Ask authors/readers for more resources
Primary effusion lymphoma (PEL) is caused by Kaposi's sarcoma-associated herpesvirus (KSHV) and frequently also harbors Epstein-Barr virus (EBV). The expression of KSHV- and EBV-encoded microRNAs (miRNAs) in PELs suggests a role for these miRNAs in latency and lymphomagenesis. Using PAR-CLIP, a technology which allows the direct and transcriptome-wide identification of miRNA targets, we delineate the target sites for all viral and cellular miRNAs expressed in PEL cell lines. The resulting data set revealed that KSHV miRNAs directly target more than 2000 cellular mRNAs, including many involved in pathways relevant to KSHV pathogenesis. Moreover, 58% of these mRNAs are also targeted by EBV miRNAs, via distinct binding sites. In addition to a known viral analog of cellular miR-155, we show that KSHV encodes a viral miRNA that mimics cellular miR-142-3p function. In summary, this study identifies an extensive list of KSHV miRNA targets, which are likely to influence viral replication and pathogenesis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available