Journal
CELL HOST & MICROBE
Volume 7, Issue 5, Pages 412-419Publisher
CELL PRESS
DOI: 10.1016/j.chom.2010.04.004
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Funding
- National Institutes of Health [P01 A1063302, AI27655]
- American Cancer Society [PF-07-066-01-LIB]
- Aduro BioTech
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A host defense strategy against pathogens is the induction of cell death, thereby eliminating the pathogen's intracellular niche. Pyroptosis, one such form of cell death, is dependent on inflammasome activation. In a genetic screen to identify Listeria monocyto genes mutants that induced altered levels of host cell death, we identified a mutation in Imo2473 that caused hyperstimulation of IL-1 beta secretion and pyroptosis following bacteriolysis in the macrophage cytosol. In addition, strains engineered to lyse in the cytosol by expression of both bacteriophage holin and lysin or induced to lyse by treatment with ampicillin stimulated pyroptosis. Pyroptosis was independent of the NIrp3 and NIrc4 inflammasome receptors but dependent on the inflammasome adaptor ASC and the cytosolic DNA sensor AIM2. Importantly, wild-type L. monocytogenes were also found to lyse, albeit at low levels, and trigger AIM2-dependent pyroptosis. These data suggested that pyroptosis is triggered by bacterial DNA released during cytosolic lysis.
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