Journal
CELL HOST & MICROBE
Volume 5, Issue 3, Pages 298-307Publisher
CELL PRESS
DOI: 10.1016/j.chom.2009.02.001
Keywords
-
Categories
Funding
- National Institutes of Health [AI080122, AI062773, DK043351, AI069939, DK078772]
- Massachusetts Biomedical Research Corporation
- American Gastroenterological Association
- American Liver Foundation
Ask authors/readers for more resources
Hepatitis C virus (HCV) chronically infects 3% of the world's population, and complications from HCV are the leading indication for liver transplantation. Given the need for better anti-HCV therapies, one strategy is to identify and target cellular cofactors of the virus lifecycle. Using a genome-wide si RNA library, we identified 96 human genes that support HCV replication, with a significant number of them being involved in vesicle organization and biogenesis. Phosphatidylinositol 4-kinase PI4KA and multiple subunits of the COPI vesicle coat complex were among the genes identified. Consistent with this, pharmacologic inhibitors of COPI and PI4KA blocked HCV replication. Targeting hepcidin, a peptide critical for iron homeostasis, also affected HCV replication, which may explain the known dysregulation of iron homeostasis in HCV infection. The host cofactors for HCV replication identified in this study should serve as a useful resource in delineating new targets for anti-HCV therapies.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available