Journal
CELL DEATH AND DIFFERENTIATION
Volume 20, Issue 1, Pages 64-76Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/cdd.2012.93
Keywords
Annexin V; phosphatidylserine; membrane vesicles
Categories
Funding
- Mayo Foundation for Education and Research
- [R01 CA69008]
- NATIONAL CANCER INSTITUTE [R01CA069008, P30CA015083] Funding Source: NIH RePORTER
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Phosphatidylserine (PS) exposure on the external leaflet of the plasma membrane is widely observed during apoptosis and forms the basis for the annexin V binding assay to detect apoptotic cell death. Current efforts to explain PS exposure focus on two potential mechanisms, activation of a phospholipid scramblase or calcium-mediated trafficking of lysosomes to the cell surface. Here, we provide evidence that apoptotic PS exposure instead reflects bidirectional trafficking of membrane between the cell surface and cytoplasm. Using a series of cell lines, some of which expose large amounts of PS during apoptosis and some of which do not, we demonstrate that accumulation of plasma membrane-derived cytoplasmic vesicles in a dynamin-, clathrin- and Cdc42-independent manner is a previously undescribed but widely occurring feature of apoptosis. The apoptotic exposure of PS occurs when these vesicles traffic back to cell surface in a calcium-dependent process that is deficient in a substantial fraction of human cancer cell lines. These observations provide a new model for PS externalization during apoptosis and simultaneously identify an altered step that accounts for the paucity of apoptotic PS exposure in many cell lines. Cell Death and Differentiation (2013) 20, 64-76; doi:10.1038/cdd.2012.93; published online 3 August 2012
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