Journal
CELL DEATH AND DIFFERENTIATION
Volume 19, Issue 6, Pages 926-936Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/cdd.2011.173
Keywords
MAGE; PML; sumoylation; acetylation; p53; senescence
Categories
Funding
- Associazione Italiana per la Ricerca sul Cancro (AIRC) [IG4752, 2006053543_003]
- ANPCyT [PICT07/00283]
- CONICET [PIP/0674]
- AREA Science Park
Ask authors/readers for more resources
MAGE-A genes are a subfamily of the melanoma antigen genes (MAGEs), whose expression is restricted to tumor cells of different origin and normal tissues of the human germline. Although the specific function of individual MAGE-A proteins is being currently explored, compelling evidence suggest their involvement in the regulation of different pathways during tumor progression. We have previously reported that MageA2 binds histone deacetylase (HDAC)3 and represses p53-dependent apoptosis in response to chemotherapeutic drugs. The promyelocytic leukemia (PML) tumor suppressor is a regulator of p53 acetylation and function in cellular senescence. Here, we demonstrate that MageA2 interferes with p53 acetylation at PML-nuclear bodies (NBs) and with PMLIV-dependent activation of p53. Moreover, a fraction of MageA2 colocalizes with PML-NBs through direct association with PML, and decreases PMLIV sumoylation through an HDAC-dependent mechanism. This reduction in PML post-translational modification promotes defects in PML-NBs formation. Remarkably, we show that in human fibroblasts expressing RasV12 oncogene, MageA2 expression decreases cellular senescence and increases proliferation. These results correlate with a reduction in NBs number and an impaired p53 response. All these data suggest that MageA2, in addition to its anti-apoptotic effect, could have a novel role in the early progression to malignancy by interfering with PML/p53 function, thereby blocking the senescence program, a critical barrier against cell transformation. Cell Death and Differentiation (2012) 19, 926-936; doi:10.1038/cdd.2011.173; published online 25 November 2011
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available