Journal
JOURNAL OF BIOLOGICAL PHYSICS
Volume 29, Issue 2-3, Pages 275-286Publisher
SPRINGER
DOI: 10.1023/A:1024465414395
Keywords
Alcian blue; BrdU; colony formation; cytotoxicity; FTIR; heating; MTT; spectromicroscopy; synchrotron; viability
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Synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectromicroscopy is a newly emerging bioanalytical and imaging tool. This unique technique provides mid-infrared (IR) spectra, hence chemical information, with high signal-to-noise at spatial resolutions as fine as 3 to 10 microns. Thus it enables researchers to locate, identify, and track specific chemical events within an individual living mammalian cell. Mid-IR photons are too low in energy (0.05-0.5 eV) to either break bonds or to cause ionization. In this review, we show that the synchrotron IR beam has no detectable effects on the short- and long-term viability, reproductive integrity, cell-cycle progression, and mitochondrial metabolism in living human cells, and produces only minimal sample heating (<0.5 degrees C). These studies have established an important foundation for SR-FTIR spectromicroscopy in biological and biomedical research.
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