Journal
CELL DEATH AND DIFFERENTIATION
Volume 18, Issue 7, Pages 1161-1173Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/cdd.2010.184
Keywords
T lymphocytes; diacylglycerol kinase alpha; multivesicular bodies; Fas ligand; exosomes
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Funding
- Ministerio de Ciencia e Innovacion [BFU2007-61613, BFU2010-18726]
- Spanish Ministerio de Ciencia y Tecnologia
- Ramon y Cajal Program
- UK Medical Research Council
- AECC
- Medical Research Council [MC_U122665002] Funding Source: researchfish
- MRC [MC_U122665002] Funding Source: UKRI
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Multivesicular bodies (MVBs) are endocytic compartments that contain intraluminal vesicles formed by inward budding from the limiting membrane of endosomes. In T lymphocytes, these vesicles contain pro-apoptotic Fas ligand (FasL), which may be secreted as 'lethal exosomes' upon fusion of MVBs with the plasma membrane. Diacylglycerol kinase alpha (DGK alpha) regulate the secretion of exosomes, but it is unclear how this control is mediated. T-lymphocyte activation increases the number of MVBs that contain FasL. DGK alpha is recruited to MVBs and to exosomes in which it has a double function. DGK alpha kinase activity exerts a negative role in the formation of mature MVBs, as we demonstrate by the use of an inhibitor. Downmodulation of DGK alpha protein resulted in inhibition of both the polarisation of MVBs towards immune synapse and exosome secretion. The subcellular location of DGK alpha together with its complex role in the formation and polarised traffic of MVBs support the notion that DGK alpha is a key regulator of the polarised secretion of exosomes. Cell Death and Differentiation (2011) 18, 1161-1173; doi:10.1038/cdd.2010.184; published online 21 January 2011
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