Journal
RECEPTORS & CHANNELS
Volume 9, Issue 1, Pages 13-17Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/10606820308257
Keywords
gigaseal; high throughput electrophysiology; ion channel; patch clamp automation; whole cell recording
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The race for creating an automated patch clamp has begun. Here, we present a novel technology to produce true gigaseals and whole cell preparations at a high rate. Suspended cells are flushed toward the tip of glass micropipettes. Seal, whole-cell break-in, and pipette/liquid handling are fully automated. Extremely stable seals and access resistance guarantee high recording quality. Data obtained from different cell types sealed inside pipettes show long-term stability, voltage clamp and seal quality, as well as block by compounds in the pM range. A flexible array of independent electrode positions minimizes consumables consumption at maximal throughput. Pulled micropipettes guarantee a proven gigaseal substrate with ultraclean and smooth surface at low cost.
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