Journal
JOURNAL OF FISH BIOLOGY
Volume 86, Issue 5, Pages 1650-1657Publisher
WILEY-BLACKWELL
DOI: 10.1111/jfb.12669
Keywords
Bayesian methods; cross-species amplification; genetic markers; genomic library; power analysis
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Funding
- BCLME/BENEFIT
- Department of Science and Technology African Coelacanth Ecosystem Programme (ACEP) II
- ACEP II
- University of Pretoria's postdoctoral programme
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The utility of 15 new and 17 previously published microsatellite markers was evaluated for species identification and stock delimitation in the deep-water hake Merluccius paradoxus and the shallow-water hake Merluccius capensis. A total of 14 microsatellites were polymorphic in M. paradoxus and 10 in M. capensis. Two markers could individually discriminate the species using Bayesian clustering methods and a statistical power analysis showed that the set of markers for each species is likely to detect subtle genetic differentiation (F-ST < 0006) that will be valuable to delimit and characterize genetic stocks.
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