Journal
CELL CYCLE
Volume 13, Issue 5, Pages 739-748Publisher
TAYLOR & FRANCIS INC
DOI: 10.4161/cc.27549
Keywords
senescence; heart; cardiac progenitor cell; CENP-A; cell cycle
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Funding
- National Heart, Lung, and Blood Institute [1R37HL091102, 1R01HL105759, 5R01HL067245, 1R01HL113656, 1R01HL117163, 1R01HL113647]
- American Heart Association [12POST12060191]
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Centromere protein A (CENP-A) is a homolog of histone H3 that epigenetically marks the heterochromatin of chromosomes. CENP-A is a critical component of the cell cycle machinery that is necessary for proper assembly of the mitotic spindle. However, the role of CENP-A in the heart and cardiac progenitor cells (CPCs) has not been previously studied. This study shows that CENP-A is expressed in CPCs and declines with age. Silencing CENP-A results in a decreased CPC growth rate, reduced cell number in phase G(2)/M of the cell cycle, and increased senescence associated beta-galactosidase activity. Lineage commitment is not affected by CENP-A silencing, suggesting that cell cycle arrest induced by loss of CENP-A is a consequence of senescence and not differentiation. CENP-A knockdown does not exacerbate cell death in undifferentiated CPCs, but increases apoptosis upon lineage commitment. Taken together, these results indicate that CPCs maintain relatively high levels of CENP-A early in life, which is necessary for sustaining proliferation, inhibiting senescence, and promoting survival following differentiation of CPCs.
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