Journal
CELL CYCLE
Volume 11, Issue 20, Pages 3876-3886Publisher
TAYLOR & FRANCIS INC
DOI: 10.4161/cc.22073
Keywords
mitochondria; dj-1 beta; oxidative stress; Drosophila; lifespan
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Funding
- Academy of Finland
- Tampere University Hospital Medical Research Fund
- European Research Council
- Spanish Ministry of Science and Innovation
- Autonomous Government of Catalonia
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DJ-1 (or PARK-7) is a multifunctional protein implicated in numerous pathologies including cancer, sterility and Parkinson disease (PD). The popular genetic model Drosophila melanogaster has two orthologs, dj-1: alpha and beta. Dysfunction of dj-1 beta strongly impairs fly mobility in an age-dependent manner. In this study, we analyze in detail the molecular mechanism underlying the dj-1 beta mutant phenotype. Mitochondrial hydrogen peroxide production, but not superoxide production, was increased in mutant flies. An increase in peroxide leak from mitochondria causes oxidative damage elsewhere and explains the strong reduction in mobility caused by dj-1 beta mutation. However, at the same time, increased levels of hydrogen peroxide activated a pro-survival program characterized by (1) an alteration in insulin-like signaling, (2) an increase in mitochondrial biogenesis and (3) an increase in the de-acetylase activity of sirtuins. The activation of this pro-survival program was associated with increased longevity under conditions of moderate oxidative stress. Additionally, the dj-1 alpha mutation unexpectedly accelerated development, a phenotype not previously associated with this mutation. Our results reveal an important role of dj-1 beta in oxidative stress handling, insulin-like signaling and development in Drosophila melanogaster.
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