Journal
CELL CYCLE
Volume 11, Issue 7, Pages 1414-1431Publisher
TAYLOR & FRANCIS INC
DOI: 10.4161/cc.19823
Keywords
REIC; gastric carcinoma; carcinogenesis; progression; prognosis
Categories
Funding
- Shenyang Outstanding Talent Foundation of China
- Shenyang Science and Technology [121561, 120103, F10-149-9-25, F11-264-1-10]
- Liaoning Science and Technology Grant
- National Natural Scientific Foundation of China [81001093, 81101885, 81101886, 81172371]
- Liaoning Provincial Education Department [L2010649]
- Specialized Research Fund for the Doctoral Program of Higher Education [20102104120026]
- Ministry of Education, Culture, Sports and Technology of Japan [23659958]
Ask authors/readers for more resources
REIC is downregulated in immortalized cell lines compared with the parental normal counterparts. It may inhibit colony formation, tumor growth and induce apoptosis. Here, gastric carcinoma or epithelial cells transfected with REIC-expressing plasmid, its siRNA or treated with recombinant REIC were subjected to the phenotypes' measurement or related molecules' detection. REIC expression was examined in gastric carcinomas by RT-PCR, western blot and immunohistochemistry. REIC overexpression or treatment resulted in a low karyoplasmic ratio and proliferation, G(1) arrest, high apoptosis, low migration, invasion or lamellipodia formation in AGS cells. REIC knockdown caused the opposite in GES-1 cells. Anti-REIC antibody blocked the effects of REIC overexpression on proliferation, G(1)/S progression and apoptosis. Ectopic REIC expression downregulated the expression of beta-catenin, phosphorylated S6K (Thr389), phosphorylated Akt1/2/3 (Ser473), cyclin D2 and E, WAVE2 and upregulated phosphorylated mTOR (Ser2448) expression and the mRNA level of Akt1, Akt2, mTO R, Raptor and Rictor in AGS cells. REIC expression was negatively associated with tumor size, lymph node metastasis, dedifferentiation or poor prognosis of carcinoma. The serum REIC level was significantly higher in healthy individuals than the carcinoma patients and inversely linked to tumor size by ELISA. The possible mechanisms underlying the forced REIC overexpression or recombinant REIC mediated the reversal of the aggressive phenotypes of gastric carcinoma cells are to downregulate beta-catenin and WAVE2 expression and to alter other related target proteins. Downregulated REIC expression was closely linked to aggressive behaviors and poor prognosis of gastric carcinoma.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available