Journal
CELL CYCLE
Volume 11, Issue 15, Pages 2931-2946Publisher
LANDES BIOSCIENCE
DOI: 10.4161/cc.21396
Keywords
BRCA1; centrosome; centriole; microtubules; aster formation
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Funding
- National Institute of Health [GM087328]
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BRCA1, a product of a familial breast and ovarian cancer susceptibility gene, localizes to centrosomes and physically interacts with gamma-tubulin, a key centrosomal protein for microtubule nucleation and anchoring at centrosomes. Here, we performed a rigorous analysis of centrosome localization of BRCA1, and found that BRCA1 is specifically associated with mother centrioles in unduplicated centrosomes, and daughter centrioles acquire BRCA1 prior to initiation of duplication, and thus duplicated centrosomes are both bound by BRCA1. We further found that BRCA1 suppresses centrosomal aster formation. In addition, we identified a new domain of BRCA1 critical for gamma-tubulin binding, which confers not only its localization to centrosomes, but also its activity to suppress centrosomal aster formation.
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