Journal
BIOPHYSICAL JOURNAL
Volume 84, Issue 1, Pages 578-587Publisher
CELL PRESS
DOI: 10.1016/S0006-3495(03)74878-4
Keywords
-
Categories
Ask authors/readers for more resources
The efficiencies of the binary liposomes composed of 1,2-dimyristoyl-sn-glycero-3-phosphocholine and cationic gemini surfactant, (2S,3R)-2,3-dimethoxy-1,4-bis(N-hexadecyl-N,N-dimethylammonium)butane dibromide as transfection vectors, were measured using the enhanced green fluorescent protein coding plasmid and COS-1 cells. Strong, correlation between the transfection efficiency and lipid stoichiometry was observed. Accordingly, liposomes with XSR-1 greater than or equal to 0.50 conveyed. the enhanced green fluorescent protein coding plasmid effectively into cells. The condensation of DNA by liposomes with XSR-1 > 0.50 was indicated by static light scattering and ethidium bromide intercalation assay, whereas differential scanning calorimetry and fluorescence anisotropy of diphenylhexatriene revealed stoichiometry dependent reorganization in the headgroup region of the liposome bilayer, in alignment with our previous Langmuir-balance study. Surface charge density and the organization of positive charges appear to determine the mode of interaction of DNA with (2S,3R)-2,3-dimethoxy-1,4-bis(N-hexadecyl-N,N-dimethylammonium)butane dibromide/1,2-dimyristoyl-sn-glycero-3-phosphocholine liposomes, only resulting in DNA condensation when XSR-1 > 0.50. Condensation of DNA in turn seems to be required for efficient transfection.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available