Journal
JOURNAL OF HEPATOLOGY
Volume 39, Issue 5, Pages 786-792Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0168-8278(03)00395-7
Keywords
telomere; telomerase; dysplastic nodule; hepatocellular carcinoma; large regenerative nodule; hepatocarcinogenesis; cirrhosis
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Backgrounds/Aims: The maintenance of telomere with telomerase reactivation, vital for carcinogenesis, was studied in human multistep hepatocarcinogenesis for the characterization of borderline lesions. Methods: The terminal restriction fragment length (TRFL) and telomerase activity (TA) were examined in 3 chronic hepatitis (CH), 10 cirrhosis, 7 large regenerative nodules (LRNs), 30 low grade dysplastic nodules (LGDNs), 6 high grade DNs (HGDNs), 3 DNs with hepatocellular carcinoma (HCC) foci, 11 HCCs, and 4 normal livers by Southern hybridization and TRAPeze Elisa telomerase detection. Results: The TRFL and TA showed significant differences between the LGDNs and HGDNs. Most LGDNs had similar levels of TRFL and TA to those of the CH, cirrhosis and LRNs, however, 17% of LGDNs revealed shortening of telomeres up to the levels of HGDNs and 7% of LGDNs showed high levels of TA. The levels of TRFL and TA in HGDNs showed no significant differences from those of DNs with HCC foci and HCCs. Conclusions: The shortening of telomeres and reactivation of telomerase occur in the DNs during the early stages of hepatocarcinogenesis, with a significant change in the transition of LGDNs to HGDNs. The characteristics of HGDNs are considered to be closer to those of HCCs. (C) 2003 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.
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