Journal
CELL CYCLE
Volume 8, Issue 10, Pages 1616-1620Publisher
TAYLOR & FRANCIS INC
DOI: 10.4161/cc.8.10.8577
Keywords
aneuploidy; cell synchronization; citrate synthase; LC3; lysosomal inhibitor; mitochondrial mass; mitophagosome; mitotic cell death; mitotic spindle; nocodazole
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Funding
- Department of Defense New Investigator Award [W81XWH-08-1-0475]
- Public Health Service [CA59971]
- John S. Dunn Research Foundation (W.L.M.) [DK35310]
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From microscopic observations of autophagosome content it has been argued that autophagy is shut down during mitosis to protect the relative short-lived organelles spindle and chromosomes from the process while they are contiguous with cytosol. However, without autophagy, buildup of dysfunctional mitochondria arising from the intense energy demands of mitosis potentially poses a hazard to accurate partition of chromosomes. Here we show using biochemical markers of autophagosomes and mitophagosomes and a blockade at the lysosomal clearance step that autophagy/mitophagy persists during mitosis at robust levels equal to interphase. This suggests a mechanism that insulates normal spindle and chromosomes from autophagy and potentially recognition of defects in spindle and chromosomes by the autophagic process.
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