Journal
CELL CYCLE
Volume 7, Issue 17, Pages 2705-2709Publisher
LANDES BIOSCIENCE
DOI: 10.4161/cc.7.17.6551
Keywords
nek6; DNA damage checkpoint; G(2)/M arrest; protein phosphorylation; chk1; chk2
Categories
Funding
- Korean Government [KRF-2006-331-E00026]
- KOSEF (Korea Science and Engineering Foundation) [M1075604000107N560400110]
Ask authors/readers for more resources
Nek6 is a recently identified NIMA-related kinase that is required for mitotic cell cycle progression. In the present study, we examined the role of Nek6 in the DNA damage response. We found that Nek6 is phosphorylated upon IR and UV irradiation through the DNA damage checkpoint in vivo. Nek6 is also directly phosphorylated by the checkpoint kinases Chk1 and Chk2 in vitro. Notably, Nek6 activation during mitosis is completely abolished by IR and UV irradiation. Moreover, the ectopic expression of Nek6 overrides DNA damage-induced G(2)/M arrest. These results suggest that Nek6 is a novel target of the DNA damage checkpoint and that the inhibition of Nek6 activity is required for proper cell cycle arrest in the G(2)/M phase upon DNA damage.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available