Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 69, Issue 11, Pages 6698-6702Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.69.11.6698-6702.2003
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Funding
- NIAID NIH HHS [AI 42708] Funding Source: Medline
- NIGMS NIH HHS [R01 GM067937, GM067937] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM067937] Funding Source: NIH RePORTER
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The medicinal value associated with complex polyketide and nonribosomal peptide natural products has prompted biosynthetic schemes dependent upon heterologous microbial hosts. Here we report the successful biosynthesis of yersiniabactin (Ybt), a model polyketide-nonribosomal peptide hybrid natural product, using Escherichia coli as a heterologous host. After introducing the biochemical pathway for Ybt into E. coli, biosynthesis was initially monitored qualitatively by mass spectrometry. Next, production of Ybt was quantified in a high-cell-density fermentation environment with titers reaching 67 +/- 21 (mean standard deviation) mg/liter and a volumetric productivity of 1.1 +/- 0.3 mg/liter-h. This success has implications for basic and applied studies on Ybt biosynthesis and also, more generally, for future production of polyketide, nonribosomal peptide, and mixed polyketide-nonribosomal peptide natural products using E. coli.
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