Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 69, Issue 11, Pages 6949-6953Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.69.11.6949-6953.2003
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Funding
- NIGMS NIH HHS [GM42893, R01 GM042893] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM042893, R29GM042893] Funding Source: NIH RePORTER
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An ultrasensitive bioassay system for the detection of N-acylhomoserine lactones (AHLs) was constructed in Agrobacterium tumefaciens by using the T7 expression system to overproduce the AHL receptor TraR. This strain detected many diverse AHLs, some at extremely low concentrations. We used this strain to detect for the first time AHLs made by Mesorhizobium huakuii, which symbiotically fixes nitrogen in association with the legume Astragalus sinicus, a source of green manure throughout eastern Asia.
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