Journal
BIOPOLYMERS
Volume 70, Issue 4, Pages 604-613Publisher
WILEY-BLACKWELL
DOI: 10.1002/bip.10505
Keywords
attenuated total reflectance; docosahexanoic acid; Fourier transform IR spectroscopy; polyunsaturated fatty acid
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The aim of this study was to develop a nondestructive method to quantitate relative amounts of n-3 and n-6 polyunsaturated fatty acid (PUFA) species in vegetable oils and oil seeds using Fourier transform IR spectroscopy (FTIR). The alkene C-H stretching vibrations of unsaturated fatty acids in oils showed IR absorption bands with various peak positions and intensities at around 3010 cm(-1), depending on the extent of unsaturation and PUFA species. With the aid of partial least-squares regression analysis, the FTIR measurement could practically predict the content of each PUFA species in the oil to be tested. A calculation method was also presented to directly find PUFA species in oils from the FTIR spectra. This technique was applied to dried soybean seeds to demonstrate a nonhomogenous distribution of saturated fatty acids and PUFAs, as well as glycans, in soybean cross sections. (C) 2003 Wiley Periodicals, Inc.
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