Journal
CELL CALCIUM
Volume 45, Issue 5, Pages 509-516Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ceca.2009.03.014
Keywords
Mitochondria; Patch-clamp; Inner membrane; IK(Ca); HCT116
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Funding
- Italian Association for Cancer Research (AIRC)
- FIRB grant (Italian Foundation for Basic Research)
- Fondazione Cassa Risparmio Perugia
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Patch-clamping mitoplasts isolated from human colon carcinoma 116 cells has allowed the identification and characterization of the intermediate conductance Ca2+-activated K+-selective channel K(Ca)3.1, previously studied only in the plasma membrane of various cell types. Its identity has been established by its biophysical and pharmacological properties. Its localisation in the inner membrane of mitochondria is indicated by Western blots of subcellular fractions, by recording of its activity in mitochondria made fluorescent by a mitochondria-targeted fluorescent protein and by the co-presence of channels considered to be markers of the inner membrane. Moderate increases of mitochondrial matrix [Ca2+] will cause mtK(Ca)3.1 opening, thus linking inner membrane K+ permeability and transmembrane potential to Ca2+ signalling. (C) 2009 Elsevier Ltd. All rights reserved.
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