Journal
CELL BIOLOGY INTERNATIONAL
Volume 38, Issue 5, Pages 591-598Publisher
WILEY
DOI: 10.1002/cbin.10235
Keywords
ERK1/2; PI3K/Akt; interleukin-6; osteocyte; compressive force
Categories
Funding
- National Natural Science Foundation of China [81170941]
- Science & Technology Support Project, Science and Technology Department of the Sichuan Province [2011SZ0157]
- National University Student Innovation Program [201210610097]
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IL-6 has a dual role in bone remodelling. The ERK1/2 pathway partially upregulated IL-6 secretion in osteocyte-like MLO-Y4 cells exposed to CCF. We have now investigated the possible role of phosphatidylinositol 3-kinase (PI3K)/Akt signalling pathway in the CCF-induced IL-6 expression. MLO-Y4 cells were treated with CCF 2,000 mu strain, 2 Hz, or 10, 30 min, 1, 3 and 6 h. IL-6 expression, Akt and ERK1/2 and PI3K/Akt phosphorylation were determined by RT-PCR, ELISA and Western blotting. Inhibition of PI3K/Akt with LY294002 or ERK1/2 with PD98059 significantly attenuated IL-6 upregulation, and IL-6 expression was abolished by inhibiting both pathways. Inhibition of one pathway downregulated the other's phosphorylation level. In conclusion, concomitant activation of PI3K/Akt and ERK1/2 pathways mediated IL-6 expression in MLO-Y4 cells under CCF.
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