Journal
CELL BIOLOGY INTERNATIONAL
Volume 37, Issue 9, Pages 995-1002Publisher
WILEY
DOI: 10.1002/cbin.10116
Keywords
3D matrices; array; assay; cell locomotion; electrotaxis; morphology
Categories
Funding
- NSF [MCB-0951199]
- California Institute of Regenerative Medicine [RB1-01417]
- NIH [1R01EY019101]
- Research to Prevent Blindness, UC Davis Ophthalmology
- Yunnan Province Talented Recruiting Program [2009CI127]
- National Science Foundation of China [U1132603]
- Direct For Biological Sciences [0951199] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience [0951199] Funding Source: National Science Foundation
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Cell behaviour in 3D environments can be significantly different from those in 2D cultures. With many different 3D matrices being developed and many experimental modalities used to modulate cell behaviour in 3D, it is necessary to develop high throughput techniques to study behaviour in 3D. We report on a 3D array on slide and have adapted this to our electrotaxis chamber, thereby offering a novel approach to quantify cellular responses to electric fields (EFs) in 3D conditions, in different matrices, with different strains of cells, under various field strengths. Dictyostelium cells to illustrate possible applications and limitations.
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