Journal
CELL BIOLOGY INTERNATIONAL
Volume 37, Issue 3, Pages 250-256Publisher
WILEY
DOI: 10.1002/cbin.10033
Keywords
cell cycle arrest; mesenchymal stem cells; placenta; umbilical cord; T cells
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Funding
- Universiti Putra Malaysia [04-01-09-0781RU]
- Ministry of Science, Technology and Innovation (MOSTI), Malaysia [02-01-04-SF1022]
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Mesenchymal stem cells (MSC) generated from human umbilical cord (UC-MSC) and placenta (PLC-MSC) were assessed and compared for their immunomodulatory function on T cells proliferation by analysis of the cell cycle. Mitogen stimulated or resting T cells were co-cultured in the presence or absence of MSC. T-cell proliferation was assessed by tritiated thymidine (3H-TdR) assay and the mechanism of inhibition was examined bycell cycle and apoptosis assay. Both UC-MSC and PLC-MSC profoundly inhibited the proliferation of T-cell, mainly via cell-to-cell contact. MSC-mediated anti-proliferation does not lead to apoptosis,but prevented T cells from entering S phase and they therefore accumulated in the G0/G1 phases. The anti-proliferative activity of MSC was related to this cell cycle arrest of T-cell. UC-MSC produced a greater inhibition than PLC-MSC in all measured parameters.
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