Journal
CELL BIOLOGY INTERNATIONAL
Volume 36, Issue 10, Pages 917-922Publisher
WILEY
DOI: 10.1042/CBI20120037
Keywords
cancer-testis antigen; mesenchymal stem cells; OY-TES-1
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Funding
- National Natural Science Foundation of China [30360026, 30760055]
- Guangxi Educational Bureau [2010105981001D34]
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OY-TES-1 is a member of the CTA (cancer-testis antigen) group expressed in a variety of cancer and restrictedly expressed in adult normal tissues, except for testis. To determine whether MSCs (mesenchymal stem cells) express OY-TES-1 and its possible roles on MSCs, OY-TES-1 expression in MSCs isolated from human bone marrow was tested with RT (reverse transcription)-PCR, immunocytochemistry and Western blot. Using RNAi (RNA interference) technology, OY-TES-1 expression was knocked down followed by analysing cell viability, cell cycle, apoptosis and migration ability. MSCs expressed OY-TES-1 at both mRNA and protein levels. The down-regulation of OY-TES-1 expression in these MSCs caused cell growth inhibition, cell cycle arrest, apoptosis induction and migration ability attenuation. Through these primary results it was suggested that OY-TES-1 may influence the biological behaviour of MSCs.
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