Journal
CELL BIOLOGY INTERNATIONAL
Volume 34, Issue 9, Pages 925-931Publisher
PORTLAND PRESS LTD
DOI: 10.1042/CBI20090457
Keywords
aging; chromatin extensibility; development; nuclear matrix; rDNA; telomere
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Funding
- Brazilian National Council for Research and Development (CNPq) [470587/03-2]
- Sao Paulo State Research Foundation (FAPESP) [2006/00066-8]
- Brazilian Research and Teaching Foundation (CAPES)
- CNPq
- CAPES
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Transcribed sequences have been suggested to be associated with the nuclear matrix, differing from non-transcribing sequences, which have been reported to be contained in DNA loops. However, although a dozen of genes have their expression level affected by aging, data on chromatin-nuclear matrix interactions under this physiological condition are still scarce. In the present study, liver imprints from young, adult and old mice were subjected to FISH (fluorescence in situ hybridization) for 45S rDNA and telomeric sequences, with or without a lysis treatment to produce extended chromatin fibres. There was an increased amount of 45S rDNA sequences located in DNA loops as the animals grow older, while telomeric sequences were always observed in DNA loops irrespective of the animal age. We assume that active rRNA genes associate with the nuclear matrix, while DNA loops contain silent sequences. Transcription of each 45S rDNA repeat unit is suggested to be dependent on its interaction with the nuclear matrix.
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