Journal
BIOPOLYMERS
Volume 72, Issue 6, Pages 413-420Publisher
WILEY
DOI: 10.1002/bip.10484
Keywords
fluorescent probe; Ph-II; phosphodiester; hydrolysis
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A new fluorescent probe for Pb-II, p-nitrophenyl 3H-phenoxazin-3-one-7-yl phosphoric acid (NPPA), was designed and synthesized by linking resorufin (serving as a fluorophore and electron acceptor) to p-nitrophenol (serving as a fluorescence quencher and electron donor) through phosphodiester bonds. When NPPA was irradiated with light, intramolecular fluorescence self-quenching took place because of the photoinduced electron transfer from the donor to the acceptor. However, upon the addition of Pb-II, the phosphate ester bonds in the probe were cleaved and the fluorophore was released, accompanying the retrievement of fluorescence. (C) 2003 Wiley Periodicals, Inc.
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