Journal
INTERNATIONAL JOURNAL OF CANCER
Volume 107, Issue 2, Pages 238-243Publisher
WILEY-LISS
DOI: 10.1002/ijc.11391
Keywords
non-small cell lung cancer; TDE1; TDE2; Tms1; Tms2; serial analysis of gene expression (SAGE); in situ hybridization
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Funding
- NATIONAL CANCER INSTITUTE [Z01CP010165, Z01CP010164] Funding Source: NIH RePORTER
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TDE2, a gene with sequence similarity to the mouse testicular tumor-differentially-expressed (Tde1/MUSTETU) gene, was identified by serial analysis of gene expression (SAGE) in nonsmall cell lung cancers (NSCLC). Here we characterized the TDE2 gene and determined its transcript levels in a panel of lung tumors, adjacent nonmalignant lung tissues and a variety of normal human tissues. In addition, we show that TDE2 is a potential transmembrane protein with 11 putative trans- membrane helices. Using real-time quantitative PCR, we showed that TDE2 transcript levels were higher in NSCLC compared to nonmalignant samples. In nonpulmonary normal tissues, the level of TDE2 was the highest in bladder, kidney and muscle; moderate to low in stomach, liver, skin, placenta and ovary tissues; and undetectable in brain, spleen and heart. By in situ hybridization, we showed that 10 of 18 lung tumors and only 1 of 14 adjacent nonmalignant regions had high levels of TDE2 transcripts. Alternatively, only 2 of 18 tumors and 8 of 14 adjacent nonmalignant bronchiole epithelium regions demonstrated negative to low levels of TDE2 signals. (C) 2003 Wiley-Liss, Inc.
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