Journal
CELL BIOLOGY INTERNATIONAL
Volume 33, Issue 11, Pages 1184-1193Publisher
WILEY
DOI: 10.1016/j.cellbi.2009.08.008
Keywords
Induced pluripotent stem cells; Embryonic stem cells; Somatic cell reprogramming; Cardiomyocytes; Differentiation
Categories
Funding
- National Institutes of Health [P20RR016464, R01HL087948]
- National Cancer Institute [R21CA131522]
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We have successfully developed both spontaneous and inductive cardiomyocyte differentiation of iPS cells reprogrammed from human foreskin fibroblasts. The reprogrammed iPS cells morphologically resemble human cardiomyocytes which can beat. RT-PCR and immunostaining show that cardiac markers are expressed that are comparable to the differentiation pattern of authentic human embryonic stem cells, indicating the existence of both immature and mature differentiated cardiomyocytes. 5-Azacytidine greatly enhanced the efficiency of cardiomyocyte differentiation, whereas dimethylsulfoxide had no effect. Low serum and bone morphogenetic protein-2 marginally improved differentiation efficiency. iPS cell-derived cardiomyocytes changed their beat frequency in response to cardiac drugs, which included ion channel blockers and alpha/beta adrenergic stimulators. Derived cardiomyocytes look promising as an in vitro system for potential drug screen and/or toxicity, making this system closer to practical use in the near future. (C) 2009 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
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