Journal
CELL BIOLOGY INTERNATIONAL
Volume 33, Issue 1, Pages 36-48Publisher
WILEY
DOI: 10.1016/j.cellbi.2008.10.001
Keywords
Exosomes; Sorting; Env protein; BLV
Categories
Funding
- Centre National de la Recherche Scientifique
- University Montpellier II
- Association pour la Recherche sur le Cancer [3444]
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Exosomes are small membrane vesicles that are released into the extracellular compartment as a consequence of fusion of multivesicular endosomes with the plasma membrane. To unravel the molecular basis of protein sorting into exosomes, we have made a chimeric protein containing the cytosolic domain of the transmembrane subunit of the viral Env protein of BLV and the ectodomain of CD8 (CDTM-BLV-CD8). When expressed in K562 cells known to constitutively secrete exosomes, the chimera was found to be very efficiently targeted to the released vesicles. Very interestingly, the cytosolic domain of the Env protein contains peptide motifs potentially recognized by components of the ESCRT machinery that could be related to chimera sorting into the vesicles. Then, quantifying the chimera secretion, we investigated the site of exosome biogenesis in K562 cells using a pharmacological approach. We present different arguments indicating that CDTM-BLV-CD8-containing exosomes are likely formed from a recycling endosomal/TGN compartment. (C) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
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