Journal
CELL BIOLOGY INTERNATIONAL
Volume 33, Issue 9, Pages 1002-1007Publisher
WILEY
DOI: 10.1016/j.cellbi.2009.05.005
Keywords
Carbonic anhydrase IX; TGF-beta; Hypoxia; Transcriptional regulation
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Funding
- Turkish Research Council (TUBITAK) [TBAG 105T326]
- Balikesir University Research Project
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Carbonic anhydrase IX (CAIX) is a membrane-associated carbonic anhydrase (CA) that is overexpressed in a variety of tumor types and associated with increased metastasis, giving a poor prognosis. Transcriptional regulation of transmembrane protein CAIX is complex. We describe further characterization of the 1.2 kb hCA9 promoter, and the effect of TGF-beta on the transcriptional activity and expression of hCAIX in Hep3B cells. Transcriptional activity of different promoter regions of hCA9 promoter showed the presence of negative regulatory region between -300 bp and -500 bp of hCAIX promoter. The -116/+38 region was enough for basal transcriptional activity in Hep3B cells. TGF-beta upregulates all promoter regions of hCA9 with the highest beig for -466/+38 that has a negatively regulated region. The transcriptional activation of hCA9 promoter by TGF-beta is consistent with hCAIX mRNA levels revealed by RT-PCR and hCAIX protein expression levels by flow cytometry in Hep3B cells. (C) 2009 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
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