Journal
NEUROSCIENCE
Volume 119, Issue 4, Pages 913-918Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0306-4522(03)00246-X
Keywords
adenosine; sleep; hypothalamus; mIPSC; presynaptic; galanin
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Funding
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [P50HL060292] Funding Source: NIH RePORTER
- NHLBI NIH HHS [P50-HL60292] Funding Source: Medline
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The ventrolateral preoptic nucleus (VLPO) is a key regulator of behavioral state that promotes sleep by directly inhibiting brain regions that maintain wakefulness. Subarachnoid administration of adenosine (AD) or AD agonists promotes sleep and induces expression of Fos protein in VLPO neurons. Therefore, activation of VLPO neurons may contribute to the somnogenic actions of AD. To define the mechanism through which AD activates VLPO neurons, we prepared hypothalamic slices from 9 to 12-day-old rat pups and recorded from 43 neurons in the galaninergic VLPO cluster; nine neurons contained galanin mRNA by post hoc in situ hybridization. Bath application of AD (20 muM) to seven of these neurons had no direct effect but caused a significant decrease in the frequency of spontaneous miniature inhibitory postsynaptic currents in the presence of tetrodotoxin, indicating a presynaptic site of action. We conclude that AD-mediated disinhibition increases the excitability of VLPO neurons thus contributing to the somnogenic properties of AD. (C) 2003 IBRO. Published by Elsevier Science Ltd. All rights reserved.
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