Journal
CELL BIOLOGY INTERNATIONAL
Volume 32, Issue 11, Pages 1439-1448Publisher
PORTLAND PRESS LTD
DOI: 10.1016/j.cellbi.2008.08.015
Keywords
Human amniotic fluid; Human bone marrow; Mesenchymal stem cells; Hepatogenic differentiation; Hepatocytes
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Funding
- Technology Project Fund of Guangdong Province, China [2003A3020303]
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Since stem cells can differentiate into hepatocyte, stem cell-based therapy becomes a potential alternative treatment for terminal liver diseases. However, an appropriate source of human mesenchymal stem cells (hMSCs) for hepatocytes has not yet been clearly elucidated. The aim of the present study was to investigate the in vitro biological characterization and hepatic differentiation potential of human amniotic fluid-derived mesenchymal stem cells (AF-hMSCs) and human bone marrow-derived mesenchymal stem cells (BM-hMSCs). Our results show that AF-hMSCs possess higher proliferation and self-renewal capacity than BM-hMSCs. Cytogenetic studies indicate that AF-hMSCs are as genetically stabile as BM-hMSCs. Following incubation with specific hepatogenic agents, AF-hMSCs showed a higher hepatic differentiation potential than BM-hMSCs. Expression of several liver-specific markers was significantly greater in AF-hMSCs than in BM-hMSCs, as shown by real time RT-PCR and immunofluorescence (IF). In conclusion, AF-hMSCs possess superior potential for hepatic differentiation, making them more suitable for diverse terminal liver diseases. (C) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
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