Journal
CELL BIOLOGY INTERNATIONAL
Volume 32, Issue 10, Pages 1256-1264Publisher
WILEY
DOI: 10.1016/j.cellbi.2008.07.010
Keywords
Mesenchymal stem cells; Human heme oxygenase-1; Gene therapy; Myocardial cell injury
Categories
Funding
- Chinese National Nature Science Foundation [30700314]
- Program for Innovative Research Team of Northeast Agricultural University [CXZ008-1]
Ask authors/readers for more resources
The aim has been to determine whether the supernatants of mesenchymal stem cells (MSCs) transfected with adenovirus carrying human heme oxygenase-1 (hHO-1) gene protect cardiomyocytes from ischemic injury. We have found that hHO-1 infected MSCs (hHO-1-MSCs) increased expression of hHO-1 protein. Apoptosis of cultured hHO-1-MSCs exposed to hypoxia was suppressed. Several cytokines, including HGF, bFGF, TGF-beta, VEGF and IL-1 beta, were produced by hHO-1-MSCs, some being significantly enhanced under hypoxia stimulation. Meanwhile, those cytokines reduced caspase-3 level and activity in cultured adult rat ventricular cardiomyocytes (ARVCs) exposed to hypoxia. Supernatants obtained from hHO-1-MSCs improved left ventricular function, limited myocardial infarct size, increased microvessel density, and inhibited apoptosis of cardiomyocytes in rat myocardial infarction. It can be concluded hHO-1-modified MSCs prevent myocardial cell injury via secretion of paracrine-acting mediators. (c) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available