Journal
CELL BIOLOGY INTERNATIONAL
Volume 32, Issue 12, Pages 1530-1535Publisher
WILEY
DOI: 10.1016/j.cellbi.2008.08.022
Keywords
Pancreatic cancer; DRGNs activity; Cell coculture; Neuron cyton size; Neurites outgrowth
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Funding
- Natural Science Foundation of Shaanxi Scientific and Technical Bureau [2006C209]
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Stimulation of mice dorsal root ganglion neurons (DRGNs) activity by human pancreatic cancer (PanCa) cell line Mia PaCa-2 and its potential molecule mechanism has been investaged. DRGNs were cultured alone or along with the MIA PaCa-2. The effects of MIA PaCa-2 to DRGNs were determined by neurofilament (NF) immunocytochemical and Nissl staining. ELISA was used to detect the concentration of insulin-like growth factor-1 (IGF-1) in the culture supernatant. Cyton size, neurite outgrowth and neuronal activity in the experimental group were greater than in the control groups. However, the concentration of IGF-1 in the supernatants was not significantly different from those in the blank and non-cultured medium groups. In the presence of MIA PaCa-2 cell line, cyton size, neurite outgrowth and neuronal activity were enhanced, which may provide more routes for the invasion of cancer cells along nerves. (c) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
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