Journal
CELL BIOLOGY INTERNATIONAL
Volume 32, Issue 8, Pages 1001-1005Publisher
ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
DOI: 10.1016/j.cellbi.2008.04.001
Keywords
Dendritic cells; Magnetic nanoparticles; Cell viability; Tumor targeting
Categories
Funding
- DGA Multidisciplinary Program
- CONSOLIDER Ingenio2010 Program
- IBERCAJA
- Ramon y Cajal program
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We have investigated the internalization of magnetic nanoparticles (NPs) into dendritic cells (DCs) in order to assess both the final location of the particles and the viability of the cultured cells. The particles, consisting of a metallic iron core covered with carbon, showed no toxic effects on the DCs and had no effect in their viability. We found that mature DCs are able to incorporate magnetic nanoparticles in a range of size from 10 nm to ca. 200 nm, after 24 h of incubation. We describe a method to separate cells loaded with NPs, and analyze the resulting material by electron microscopy and magnetic measurements. It is found that NPs are internalized in lysosomes, providing a large magnetic signal. Our results suggest that loading DCs with properly functionalized magnetic NPs could be a promising strategy for improved vectorization in cancer diagnosis and treatment. (c) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
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