Journal
CELL BIOLOGY AND TOXICOLOGY
Volume 27, Issue 2, Pages 83-94Publisher
SPRINGER
DOI: 10.1007/s10565-010-9172-4
Keywords
Geraniin; gamma-Radiation; Apoptosis; Radioprotective effect; Reactive oxygen species
Categories
Funding
- Ministry of Science & Technology (MOST) of Korea
Ask authors/readers for more resources
The radioprotective effect of geraniin, a tannin compound isolated from Nymphaea tetragona Georgi var. (Nymphaeaceae), against gamma-radiation-induced damage was investigated in Chinese hamster lung fibroblast (V79-4) cells. Geraniin recovered cell viability detected by MTT test and colony formation assay, which was compromised by gamma-radiation, and reduced the gamma-radiation-induced apoptosis by the inhibition of loss of the mitochondrial membrane potential. Geraniin protected cellular components (lipid membrane, cellular protein, and DNA) damaged by gamma-radiation, which was detected by lipid peroxidation, protein carbonyl formation, and comet assay. Geraniin significantly reduced the level of intracellular reactive oxygen species generated by gamma-radiation, which was detected using spectrofluorometer, flow cytometer, and confocal microscope after 2',7'-dichlorodihydrofluorescein diacetate staining. Geraniin normalized the superoxide dismutase and catalase activities, which were decreased by gamma-radiation. These results suggest that geraniin protects cells against radiation-induced oxidative stress via enhancing of antioxidant enzyme activities and attenuating of cellular damage.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available