Journal
CELL BIOCHEMISTRY AND FUNCTION
Volume 29, Issue 5, Pages 408-413Publisher
WILEY
DOI: 10.1002/cbf.1765
Keywords
hypothyroidism; hyperthyroidism; blood-biomarkers; GST; GPx; oxidative damage
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Funding
- Secretaria de Ciencia e Tecnologia do Estado do Rio Grande do Sul
- CNPq
- CAPES
- FAPERGS
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Thyroid hormones modulate haemoglobin and reactive oxygen species (ROS) production, leading to antioxidant changes. This study evaluated the antioxidant response to ROS in erythrocytes in hypothyroid and hyperthyroid rats. Wistar rats were divided into four groups: control; hyperthyroid (T-4-12 mg l(-1) in drinking water); sham operated (simulation of thyroidectomy); and hypothyroid (thyroidectomized). Four weeks after, blood was collected and haemoglobin and T-4 levels, lipid peroxidation (LPO), protein oxidation, superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and glutathione peroxidase (GPx) activities, and total radical antioxidant potential (TRAP) were measured. SOD, CAT and GST immunocontent was evaluated. Haemoglobin levels were increased in hyperthyroid erythrocytes. LPO and carbonyls were augmented (65% and 55%, respectively) in hyperthyroid and reduced (31% and 56%, respectively) in hypothyroid group. SOD and CAT activities have not changed, as well as CAT immunocontent. TRAP was diminished in both hyperthyroid and hypothyroid groups (36% and 37%, respectively). GST activity and immunocontent, as well as GPx activity, were increased in hyper and hypothyroid rats. The data suggest that thyroid hormone changes determine ROS concentration changes and decrease of some antioxidant defences that would lead to a compensatory answer of the GST and GPx enzymes, which could be consider as credible biomarkers. Copyright (C) 2011 John Wiley & Sons, Ltd.
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