Journal
PHYTOCHEMISTRY
Volume 64, Issue 6, Pages 1069-1076Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0031-9422(03)00507-7
Keywords
Allium cepa; Alliaceae; onion; flavonoids; quercetin; glucosyltransferases; substrate specificity; regiospecificity
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Two UDP-glucose-dependent flavonoid glucosyltransferases (EC 2,4,1,-) isolated from the epidermal layer of yellow onion (Allium cepa) were functionally expressed in Escherichia coli and their substrate specificity investigated. The two enzymes exhibited different substrate- and regio-specificity profiles. A. cepa UGT73G1 used a wide range of different flavonoid substrates including flavonoids trot naturally occurring in onion. Regiospecificity was indicated for hydroxyl-groups of the C-3, C-7 and C-4' positions of the flavan backbone structure to yield flavonoid mono- and diglucosides. In contrast, A. cepa UGT73J1 showed activity only with the flavonoid mono-glucoside isoquercitrin and the isoflavone aglycone genistein, with regiospecificity for the C-7 position. The regiospecificity for both enzymes included positions that are glucosylated in flavonoids of onion bulbs, indicating their involvement in flavonoid biosynthesis in A. cepa. (C) 2003 Elsevier Ltd. All rights reserved.
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